Protein_Domain

Part:BBa_K1778004:Design

Designed by: Lehua Jia   Group: iGEM15_Scut-Champion-Park   (2015-09-11)


rtTA is a regulatory protein which can combined with tetracycline analogues.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we use the part in pichia pastoris, we need to think that if the protein can exist stably in yeast cell and activate the promoter effectively. It need to be proved by experiment and references.


Source

rtTA is from the plasmid pTet-on, It was merged by reverse TetR and the transcriptional activation area of VP16.

References